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Environmental (e)RNA advances the reliability of eDNA by predicting its age

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Environmental (e)RNA advances the reliability of eDNA by predicting its age

Environmental DNA (eDNA) evaluation has superior conservation biology and biodiversity administration. Nevertheless, correct estimation of age and origin of eDNA is sophisticated by particle transport and the presence of legacy genetic materials, which might obscure correct interpretation of eDNA detection and quantification. To grasp the state of genomic materials inside the atmosphere, we investigated the degradation relationships between (a) measurement of fragments (lengthy vs quick), (b) genomic origins (mitochondrial vs nuclear), (c) nucleic acids (eDNA vs eRNA), and (d) RNA sorts (messenger (m)RNA vs ribosomal (r)RNA) from non-indigenous Dreissena mussels.

Preliminary concentrations of eRNA adopted anticipated transcriptional developments, with rRNAs discovered at > 1000 × that of eDNA, and a mitosis-associated mRNA falling beneath detection limits inside 24 h. Moreover, the ratio of eRNA:eDNA considerably decreased all through degradation, doubtlessly offering an estimate for the age of genomic materials. Thus, eRNA quantification can improve detection because of the excessive concentrations of rRNAs. Moreover, it might enhance interpretation of constructive detections by way of the eRNA:eDNA ratio and/or by detecting low considerable mitosis-associated mRNAs that degrade inside ~ 24 h.

Utilizing exome, genome, and Sanger sequencing, a recurrent frameshift EMC10 variant was recognized in affected people in a global cohort of consanguineous households. A number of households have been independently recognized and linked through Matchmaker Alternate and inside databases. We assessed the impact of the frameshift variant on EMC10 RNA and protein expression and evaluated EMC10 expression in regular human mind tissue utilizing immunohistochemistry.
A homozygous variant EMC10 c.287delG (Refseq NM_206538.3, p.Gly96Alafs*9) segregated with affected people in every household, who exhibited a phenotypic spectrum of mental incapacity (ID) and international developmental delay (GDD), variable seizures and variable dysmorphic options (elongated face, curly hair, cubitus valgus, and arachnodactyly). The variant arose on two founder haplotypes and ends in considerably lowered EMC10 RNA expression and an unstable truncated EMC10 protein.

New approaches to focus on RNA binding proteins

RNA binding proteins (RBPs) are a big and various class of proteins that regulate all features of RNA biology. As RBP dysregulation has been implicated in numerous human issues, together with cancers and neurodegenerative illness, small molecule chemical probes that focus on particular person RBPs characterize helpful instruments for deciphering RBP perform and guiding the manufacturing of recent therapeutics. Whereas RBPs are sometimes considered tough-to-drug, the invention of numerous small molecules that focus on RBPs has spurred appreciable latest curiosity in new methods for RBP chemical probe discovery. Right here we evaluation present and rising applied sciences for top throughput RBP-small molecule screening that we anticipate will assist unlock the complete therapeutic potential of this thrilling protein class.
Right here, we describe an in depth protocol for the isolation of purified populations of viable spermatogenic cells derived from the non-human primate mannequin organism Macaca fascicularis (cynomolgus). Utilizing fluorescence-activated cell sorting (FACS), we describe strategies to isolate spermatogonia and first spermatocytes ranging throughout the sub-stages of meiosis prophase I. These cell populations can be utilized with quite a lot of downstream assays, together with single-cell approaches equivalent to RNA sequencing, chromatin immunoprecipitation, quantitative RT-PCR, and immunocytochemistry. For full particulars on the use and execution of this protocol, please consult with Lau et al. (2020).
Environmental (e)RNA advances the reliability of eDNA by predicting its age

Abiotic Synthesis of Nucleoside 5′-Triphosphates with Nickel Borate and Cyclic Trimetaphosphate (CTMP)

Whereas nucleoside 5′-triphosphates are precursors for RNA in fashionable biology, the presumed issue of creating these triphosphates on Hadean Earth has brought about many prebiotic researchers to contemplate different activated species for the prebiotic synthesis of RNA. We report right here that nickel(II), within the presence of borate, offers substantial quantities (2-3%) of nucleoside 5′-triphosphates upon evaporative heating within the presence of urea, salts, and cyclic trimetaphosphate (CTMP). Additionally recovered are nucleoside 5′-diphosphates and nucleoside 5′-monophosphates, each seemingly arising from 5′-triphosphate intermediates.
The overall degree of 5′-phosphorylation is usually 30%. Borate enhances the regiospecificity of phosphorylation, with elevated quantities of different phosphorylated species seen in its absence. Experimentally supported paths are already out there to make nucleosides in environments more likely to have been current on Hadean Earth quickly after a midsized 1021 to 1023 kg impactor, which might even have delivered nickel to the Hadean floor. Additional, sources of prebiotic CTMP proceed to be proposed. Thus, these outcomes fill in one of many few remaining steps wanted to demystify the prebiotic synthesis of RNA and help a steady mannequin from atmospheric elements to oligomeric RNA that’s missing solely a mechanism to acquire homochirality within the product RNA.
Xanthophyllomyces dendrorhous is a basidiomycete yeast that produces carotenoids, primarily astaxanthin. Astaxanthin is an natural pigment of economic curiosity resulting from its antioxidant and coloring properties. X. dendrorhous has a purposeful SREBP pathway, and the Sre1 protein is the SREBP homolog on this yeast. Nevertheless, how sterol regulatory component (Sre)1 promotes the biosynthesis of sterols and carotenoids in X. dendrorhous is unknown. On this work, comparative RNA-sequencing evaluation between modified X. dendrorhous strains which have an lively Sre1 protein and the WT was carried out to determine Sre1-dependent genes.

Total Protein from Human Tumor Tissue: Uterus
P1235274 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Navy RNA Lysis Kit 50
NAVYE1-RNA 50pack
EUR 198
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RNA Lysis Kit 250
NAVYE5-RNA 250pack
EUR 651
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Green RNA Lysis Kit 50
GREENE1-RNA 50pack
EUR 180
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RNA Lysis Kit 250
GREENE5-RNA 250pack
EUR 571
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Pink RNA Lysis Kit 50
PINKE1-RNA 50pack
EUR 178
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RNA Lysis Kit 250
PINKE5-RNA 250pack
EUR 561
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 50
YELLOWE1-RNA 50pack
EUR 192
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 250
YELLOWE5-RNA 250pack
EUR 624
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 50
REDE1-RNA 50pack
EUR 192
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 250
REDE5-RNA 250pack
EUR 622
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E Navy RNA Lysis Kit 100
NAVY5E100-RNA 100pack
EUR 453
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

5E Navy RNA Lysis Kit 20
NAVY5E20-RNA 20pack
EUR 153
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 50
NAVYR1-RNA 50pack
EUR 202
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 250
NAVYR5-RNA 250pack
EUR 666
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

5E GREEN RNA Lysis Kit 100
GREEN5E100-RNA 100pack
EUR 386
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 1000, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

5E GREEN RNA Lysis Kit 20
GREEN5E20-RNA 20pack
EUR 138
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 50
GREENR1-RNA 50pack
EUR 184
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 250
GREENR5-RNA 250pack
EUR 587
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

5E PINK RNA Lysis Kit 100
PINK5E100-RNA 100pack
EUR 380
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E PINK RNA Lysis Kit 20
PINK5E20-RNA 20pack
EUR 136
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 50
PINKR1-RNA 50pack
EUR 181
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 250
PINKR5-RNA 250pack
EUR 576
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 50
YELLOWR1-RNA 50pack
EUR 199
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 250
YELLOWR5-RNA 250pack
EUR 656
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 100
RED5E100-RNA 100pack
EUR 429
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 20
RED5E20-RNA 20pack
EUR 148
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 50
REDR1-RNA 50pack
EUR 195
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 250
REDR5-RNA 250pack
EUR 638
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

RNA isolater Total RNA Extraction Reagent
R401-01 100 ml
EUR 133

Total Protein from Human Adult Normal Tissue: Uterus
P1234274 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA Isolation Kit
P-9100 50 samples
EUR 347.25
Description: The best epigenetics products

Total RNA Extraction Kit
K2014005 1 kit
EUR 344
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Human Total PSA (t-PSA) ELISA Kit
PRB-5049-TOTAL-5 5 x 96 assays
EUR 2283

Total Protein from Human Adult Normal Tissue: Uterus: Cervix
P1234275 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Adult Normal Tissue: Uterus: Corpus
P1234276 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Adult Normal Tissue: Uterus: Fundus
P1234278 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Rat Normal Tissue: Adipose
R1434003-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Bladder
R1434010-50 50 ug
EUR 303
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Brain
R1434035-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Colon
R1434090-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Heart
R1434122-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Kidney
R1434142-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Liver
R1434149-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Lung
R1434152-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Ovary
R1434183-50 50 ug
EUR 296
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Pancreas
R1434188-50 50 ug
EUR 296
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Placenta
R1434200-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Rectum
R1434206-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Spleen
R1434246-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Stomach
R1434248-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Testis
R1434260-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Thymus
R1434264-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA Isolation Kit (Tissue)
P-9102 50 samples
EUR 347.25
Description: reagents widely cited

Total RNA Isolation Kit (Plant)
P-9104 50 samples
EUR 347.25
Description: kits suitable for this type of research

Total RNA Isolation Kit II
FATRS--001 100 preps
EUR 308

Total RNA Isolation Kit II
FATRS--050 50 preps
EUR 216

Total RNA ExtractionMini KitAnimal Tissues
FYG301-50P 50 Preps Ask for price

Total RNA ExtractionMini KitAnimal Tissues
FYG302-100P 100 Preps Ask for price

Total RNA ExtractionMini KitAnimal Tissues
FYG302-300P 300 Preps Ask for price

Total RNA ExtractionMini KitPlant Tissues
FYG303-100P 100 Preps Ask for price

Total RNA ExtractionMini KitPlant Tissues
FYG303-300P 300 Preps Ask for price

Total RNA ExtractionMini KitPlant Tissues
FYG303-50P 50 Preps Ask for price

AnaPrep Total RNA Extraction Kit
Z1322015 1 kit (48 extractions) Including all required plastic disposables
EUR 308
Description: This kit is developped for our fully automated magnetic bead-based nucleic acid extraction platform which uses preprogrammed protocols and can process up to 12 samples simultaneously. With the AnaPrep 12 Extractor you will have the option to choose and work with a wide range of sample and reagent volumes. This sturdy, realiable and user-friendly machine will save you both time and expenses while ensuring consistently high quality performance and nucleic acids for your downstream applications.

Total RNA from Lupus: Colon
R1236090Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart
R1236122Hd-2 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Kidney
R1236142Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Liver
R1236149Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Lung
R1236152Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Pancreas
R1236188Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Spleen
R1236246Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Stomach
R1236248Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Uterus Lysate
1317 0.1 mg
EUR 191
Description: Uterus tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Total RNA from Rat Normal Tissue: Brain, cerebellum
R1434039-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Skeletal Muscle
R1434171-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Small Intestine
R1434226-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Plant Total RNA Mini Kit (50prep)
FAPRK-001 50 preps
EUR 165

Plant Total RNA Mini Kit (100prep)
FAPRK-001-1 100 preps
EUR 216

Plant Total RNA Mini Kit (300prep)
FAPRK-001-2 300 preps
EUR 404

Plant Total RNA Maxi Kit (10prep)
FAPRK-002 10 preps
EUR 201

Plant Total RNA Maxi Kit (24prep)
FAPRK-002-1 24 preps
EUR 317

Tissue Total RNA Mini Kit (50prep)
FATRK-001 50 preps
EUR 165

Tissue Total RNA Mini Kit (100prep)
FATRK-001-1 100 preps
EUR 216

Tissue Total RNA Mini Kit (300prep)
FATRK-001-2 300 preps
EUR 404

Tissue Total RNA Maxi Kit (10prep)
FATRK-003 10 preps
EUR 201

Tissue Total RNA Maxi Kit (24prep)
FATRK-003-1 24 preps
EUR 317

Total RNA ExtractionMini KitBlood/Cultured Cell
FYG301-300P 300 Preps Ask for price

Rapid Animal Total RNA Extraction Kit
AT4181 50Preps
EUR 88.06

Rapid Animal Total RNA Extraction Kit
AT4182 250preps
EUR 202.25

Zenoquick Whole Blood Total RNA Kit
Z5010-050 50 preps
EUR 482

Zenoquick Whole Blood Total RNA Kit
Z5010-100 2x50 preps
EUR 806

Broad Range Total RNA Isolation Kit
K1341050 1 kit
EUR 266
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Alzheimer's Disease: Brain
R1236035Alz-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Pons
R1236071Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Colon
R1236090Dia-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Colon
R1236090Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Esophagus
R1236106Dia-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Interventricular Septum
R1236130Hd-2 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Kidney
R1236142Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Liver
R1236149Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Lung
R1236152Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Diaphragm
R1236169Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Pancreas
R1236188Dia-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Pancreas
R1236188Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Rectum
R1236206Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Small Intestine
R1236226Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Spleen
R1236246Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Stomach
R1236248Dia-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Fetal Uterus Lysate
XBL-10431 0.1 mg
EUR 527
Description: Fetal human uterus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human uterus tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the uterus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The uterus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Uterus Membrane Lysate
XBL-11023 0.1 mg
EUR 516.5
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus Tumor Lysate
1384 0.1 mg
EUR 254
Description: Uterus tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

96-well Total RNA Kit (1 plates)
FATRE-96001 1 prep
EUR 194

96-well Total RNA Kit (2 plates)
FATRE-96002 2 preps
EUR 281

96-well Total RNA Kit (4 plates)
FATRE-96004 4 preps
EUR 448

Total RNA Plus Mini Kit (50 prep)
FATRK-P-050 50 preps
EUR 179

Total RNA Plus Mini Kit (100 prep)
FATRK-P-100 100 preps
EUR 242

Total RNA from Human Tumor Tissue: Adrenal
R1235004-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Bladder
R1235010-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Bone
R1235023-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Breast
R1235086-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Colon
R1235090-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Esophagus
R1235106-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Kidney
R1235142-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Liver
R1235149-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Lung
R1235152-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Nose
R1235178-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Ovary
R1235183-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Parotid
R1235190-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Rectum
R1235206-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Melanoma
R1235218A-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Stomach
R1235248-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Synoviosarcoma
R1235257-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Thymus
R1235264-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Thyroid
R1235265-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Tonsil
R1235268-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Corpus Callosum
R1236045Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Frontal Lobe
R1236051Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Occipital Lobe
R1236062Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Parietal Lobe
R1236066Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Postcentral Gyrus
R1236072Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Precentral Gyrus
R1236073Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
As well as, Sre1 direct goal genes have been recognized by way of ChIP mixed with lambda exonuclease digestion (ChIP-exo) assays. SRE motifs have been detected within the promoter areas of a number of Sre1 direct goal genes and have been in line with the SREs described in different yeast species. Sre1 immediately regulates genes associated to ergosterol biosynthesis in addition to genes associated to the mevalonate (MVA) pathway, which synthesizes the constructing blocks of isoprenoids, together with carotenoids. Two carotenogenic genes, crtE and crtR, have been additionally recognized as Sre1 direct goal genes.
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