Environmental DNA (eDNA) evaluation has superior conservation biology and biodiversity administration. Nevertheless, correct estimation of age and origin of eDNA is sophisticated by particle transport and the presence of legacy genetic materials, which might obscure correct interpretation of eDNA detection and quantification. To grasp the state of genomic materials inside the atmosphere, we investigated the degradation relationships between (a) measurement of fragments (lengthy vs quick), (b) genomic origins (mitochondrial vs nuclear), (c) nucleic acids (eDNA vs eRNA), and (d) RNA sorts (messenger (m)RNA vs ribosomal (r)RNA) from non-indigenous Dreissena mussels.
Preliminary concentrations of eRNA adopted anticipated transcriptional developments, with rRNAs discovered at > 1000 × that of eDNA, and a mitosis-associated mRNA falling beneath detection limits inside 24 h. Moreover, the ratio of eRNA:eDNA considerably decreased all through degradation, doubtlessly offering an estimate for the age of genomic materials. Thus, eRNA quantification can improve detection because of the excessive concentrations of rRNAs. Moreover, it might enhance interpretation of constructive detections by way of the eRNA:eDNA ratio and/or by detecting low considerable mitosis-associated mRNAs that degrade inside ~ 24 h.
Utilizing exome, genome, and Sanger sequencing, a recurrent frameshift EMC10 variant was recognized in affected people in a global cohort of consanguineous households. A number of households have been independently recognized and linked through Matchmaker Alternate and inside databases. We assessed the impact of the frameshift variant on EMC10 RNA and protein expression and evaluated EMC10 expression in regular human mind tissue utilizing immunohistochemistry.
A homozygous variant EMC10 c.287delG (Refseq NM_206538.3, p.Gly96Alafs*9) segregated with affected people in every household, who exhibited a phenotypic spectrum of mental incapacity (ID) and international developmental delay (GDD), variable seizures and variable dysmorphic options (elongated face, curly hair, cubitus valgus, and arachnodactyly). The variant arose on two founder haplotypes and ends in considerably lowered EMC10 RNA expression and an unstable truncated EMC10 protein.
New approaches to focus on RNA binding proteins
RNA binding proteins (RBPs) are a big and various class of proteins that regulate all features of RNA biology. As RBP dysregulation has been implicated in numerous human issues, together with cancers and neurodegenerative illness, small molecule chemical probes that focus on particular person RBPs characterize helpful instruments for deciphering RBP perform and guiding the manufacturing of recent therapeutics. Whereas RBPs are sometimes considered tough-to-drug, the invention of numerous small molecules that focus on RBPs has spurred appreciable latest curiosity in new methods for RBP chemical probe discovery. Right here we evaluation present and rising applied sciences for top throughput RBP-small molecule screening that we anticipate will assist unlock the complete therapeutic potential of this thrilling protein class.
Right here, we describe an in depth protocol for the isolation of purified populations of viable spermatogenic cells derived from the non-human primate mannequin organism Macaca fascicularis (cynomolgus). Utilizing fluorescence-activated cell sorting (FACS), we describe strategies to isolate spermatogonia and first spermatocytes ranging throughout the sub-stages of meiosis prophase I. These cell populations can be utilized with quite a lot of downstream assays, together with single-cell approaches equivalent to RNA sequencing, chromatin immunoprecipitation, quantitative RT-PCR, and immunocytochemistry. For full particulars on the use and execution of this protocol, please consult with Lau et al. (2020).
Abiotic Synthesis of Nucleoside 5′-Triphosphates with Nickel Borate and Cyclic Trimetaphosphate (CTMP)
Whereas nucleoside 5′-triphosphates are precursors for RNA in fashionable biology, the presumed issue of creating these triphosphates on Hadean Earth has brought about many prebiotic researchers to contemplate different activated species for the prebiotic synthesis of RNA. We report right here that nickel(II), within the presence of borate, offers substantial quantities (2-3%) of nucleoside 5′-triphosphates upon evaporative heating within the presence of urea, salts, and cyclic trimetaphosphate (CTMP). Additionally recovered are nucleoside 5′-diphosphates and nucleoside 5′-monophosphates, each seemingly arising from 5′-triphosphate intermediates.
The overall degree of 5′-phosphorylation is usually 30%. Borate enhances the regiospecificity of phosphorylation, with elevated quantities of different phosphorylated species seen in its absence. Experimentally supported paths are already out there to make nucleosides in environments more likely to have been current on Hadean Earth quickly after a midsized 1021 to 1023 kg impactor, which might even have delivered nickel to the Hadean floor. Additional, sources of prebiotic CTMP proceed to be proposed. Thus, these outcomes fill in one of many few remaining steps wanted to demystify the prebiotic synthesis of RNA and help a steady mannequin from atmospheric elements to oligomeric RNA that’s missing solely a mechanism to acquire homochirality within the product RNA.
Xanthophyllomyces dendrorhous is a basidiomycete yeast that produces carotenoids, primarily astaxanthin. Astaxanthin is an natural pigment of economic curiosity resulting from its antioxidant and coloring properties. X. dendrorhous has a purposeful SREBP pathway, and the Sre1 protein is the SREBP homolog on this yeast. Nevertheless, how sterol regulatory component (Sre)1 promotes the biosynthesis of sterols and carotenoids in X. dendrorhous is unknown. On this work, comparative RNA-sequencing evaluation between modified X. dendrorhous strains which have an lively Sre1 protein and the WT was carried out to determine Sre1-dependent genes.
 ELISA Kit) Human Total PSA (t-PSA) ELISA Kit |
|
PRB-5049-TOTAL |
Cell Biolabs |
96 assays |
EUR 572 |
 Total Protein from Human Adult Normal Tissue: Uterus |
|
P1234274 |
Biochain |
1 mg |
EUR 214 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Green RNA Lysis Kit 50 |
|
GREENE1-RNA |
Next Advance |
50pack |
EUR 180 |
|
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free. |
 Green RNA Lysis Kit 250 |
|
GREENE5-RNA |
Next Advance |
250pack |
EUR 571 |
|
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free. |
 Pink RNA Lysis Kit 50 |
|
PINKE1-RNA |
Next Advance |
50pack |
EUR 178 |
|
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
 Pink RNA Lysis Kit 250 |
|
PINKE5-RNA |
Next Advance |
250pack |
EUR 561 |
|
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
 Navy RNA Lysis Kit 50 |
|
NAVYE1-RNA |
Next Advance |
50pack |
EUR 198 |
|
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free. |
 Navy RNA Lysis Kit 250 |
|
NAVYE5-RNA |
Next Advance |
250pack |
EUR 651 |
|
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free. |
 Red RNA Lysis Kit 50 |
|
REDE1-RNA |
Next Advance |
50pack |
EUR 192 |
|
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
 Red RNA Lysis Kit 250 |
|
REDE5-RNA |
Next Advance |
250pack |
EUR 622 |
|
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
 YELLOW RNA Lysis Kit 50 |
|
YELLOWE1-RNA |
Next Advance |
50pack |
EUR 192 |
|
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
 YELLOW RNA Lysis Kit 250 |
|
YELLOWE5-RNA |
Next Advance |
250pack |
EUR 624 |
|
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
 RNA isolater Total RNA Extraction Reagent |
|
R401-01 |
Vazyme |
100 ml |
EUR 133 |
 Total RNA Isolation Kit |
|
P-9100 |
EpiGentek |
50 samples |
EUR 347.25 |
|
Description: The best epigenetics products |
 Total RNA Extraction Kit |
|
K2014005 |
Biochain |
1 kit |
EUR 344 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 5E GREEN RNA Lysis Kit 100 |
|
GREEN5E100-RNA |
Next Advance |
100pack |
EUR 386 |
|
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 1000, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free. |
 5E GREEN RNA Lysis Kit 20 |
|
GREEN5E20-RNA |
Next Advance |
20pack |
EUR 138 |
|
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free. |
 Green RINO RNA Lysis Kit 50 |
|
GREENR1-RNA |
Next Advance |
50pack |
EUR 184 |
|
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free. |
 Green RINO RNA Lysis Kit 250 |
|
GREENR5-RNA |
Next Advance |
250pack |
EUR 587 |
|
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free. |
 5E PINK RNA Lysis Kit 100 |
|
PINK5E100-RNA |
Next Advance |
100pack |
EUR 380 |
|
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
 5E PINK RNA Lysis Kit 20 |
|
PINK5E20-RNA |
Next Advance |
20pack |
EUR 136 |
|
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
 Pink RINO RNA Lysis Kit 50 |
|
PINKR1-RNA |
Next Advance |
50pack |
EUR 181 |
|
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free. |
 Pink RINO RNA Lysis Kit 250 |
|
PINKR5-RNA |
Next Advance |
250pack |
EUR 576 |
|
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free. |
 Navy RINO RNA Lysis Kit 250 |
|
NAVYR5-RNA |
Next Advance |
250pack |
EUR 666 |
|
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free. |
 5E Navy RNA Lysis Kit 100 |
|
NAVY5E100-RNA |
Next Advance |
100pack |
EUR 453 |
|
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free. |
 5E Navy RNA Lysis Kit 20 |
|
NAVY5E20-RNA |
Next Advance |
20pack |
EUR 153 |
|
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free. |
 Navy RINO RNA Lysis Kit 50 |
|
NAVYR1-RNA |
Next Advance |
50pack |
EUR 202 |
|
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free. |
 5E RED RNA Lysis Kit 100 |
|
RED5E100-RNA |
Next Advance |
100pack |
EUR 429 |
|
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
 5E RED RNA Lysis Kit 20 |
|
RED5E20-RNA |
Next Advance |
20pack |
EUR 148 |
|
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
 Red RINO RNA Lysis Kit 50 |
|
REDR1-RNA |
Next Advance |
50pack |
EUR 195 |
|
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free. |
 Red RINO RNA Lysis Kit 250 |
|
REDR5-RNA |
Next Advance |
250pack |
EUR 638 |
|
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free. |
 YELLOW RINO RNA Lysis Kit 50 |
|
YELLOWR1-RNA |
Next Advance |
50pack |
EUR 199 |
|
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free. |
 YELLOW RINO RNA Lysis Kit 250 |
|
YELLOWR5-RNA |
Next Advance |
250pack |
EUR 656 |
|
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free. |
 Total Protein from Human Adult Normal Tissue: Uterus: Cervix |
|
P1234275 |
Biochain |
1 mg |
EUR 214 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Total Protein from Human Adult Normal Tissue: Uterus: Corpus |
|
P1234276 |
Biochain |
1 mg |
EUR 214 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Total Protein from Human Adult Normal Tissue: Uterus: Fundus |
|
P1234278 |
Biochain |
1 mg |
EUR 214 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Human Total PSA (t-PSA) ELISA Kit |
|
PRB-5049-TOTAL-5 |
Cell Biolabs |
5 x 96 assays |
EUR 2283 |
 Total RNA from Rat Normal Tissue: Adipose |
|
R1434003-50 |
Biochain |
50 ug |
EUR 214 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Bladder |
|
R1434010-50 |
Biochain |
50 ug |
EUR 303 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Brain |
|
R1434035-50 |
Biochain |
50 ug |
EUR 152 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Colon |
|
R1434090-50 |
Biochain |
50 ug |
EUR 152 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Heart |
|
R1434122-50 |
Biochain |
50 ug |
EUR 152 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Kidney |
|
R1434142-50 |
Biochain |
50 ug |
EUR 152 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Liver |
|
R1434149-50 |
Biochain |
50 ug |
EUR 152 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Lung |
|
R1434152-50 |
Biochain |
50 ug |
EUR 152 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Ovary |
|
R1434183-50 |
Biochain |
50 ug |
EUR 296 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Pancreas |
|
R1434188-50 |
Biochain |
50 ug |
EUR 296 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Placenta |
|
R1434200-50 |
Biochain |
50 ug |
EUR 214 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Rectum |
|
R1434206-50 |
Biochain |
50 ug |
EUR 214 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Spleen |
|
R1434246-50 |
Biochain |
50 ug |
EUR 152 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Stomach |
|
R1434248-50 |
Biochain |
50 ug |
EUR 214 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Testis |
|
R1434260-50 |
Biochain |
50 ug |
EUR 152 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Thymus |
|
R1434264-50 |
Biochain |
50 ug |
EUR 214 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA Isolation Kit II |
|
FATRS--001 |
Favorgen |
100 preps |
EUR 308 |
Total RNA Isolation Kit II |
|
FATRS--050 |
Favorgen |
50 preps |
EUR 216 |
) Total RNA Isolation Kit (Tissue) |
|
P-9102 |
EpiGentek |
50 samples |
EUR 347.25 |
|
Description: reagents widely cited |
) Total RNA Isolation Kit (Plant) |
|
P-9104 |
EpiGentek |
50 samples |
EUR 347.25 |
|
Description: kits suitable for this type of research |
 Total RNA ExtractionMini KitAnimal Tissues |
|
FYG301-50P |
Yeastern Biotech |
50 Preps |
Ask for price |
Total RNA ExtractionMini KitAnimal Tissues |
|
FYG302-100P |
Yeastern Biotech |
100 Preps |
Ask for price |
Total RNA ExtractionMini KitAnimal Tissues |
|
FYG302-300P |
Yeastern Biotech |
300 Preps |
Ask for price |
 Total RNA ExtractionMini KitPlant Tissues |
|
FYG303-100P |
Yeastern Biotech |
100 Preps |
Ask for price |
Total RNA ExtractionMini KitPlant Tissues |
|
FYG303-300P |
Yeastern Biotech |
300 Preps |
Ask for price |
Total RNA ExtractionMini KitPlant Tissues |
|
FYG303-50P |
Yeastern Biotech |
50 Preps |
Ask for price |
 Total RNA from Lupus: Colon |
|
R1236090Lup-50 |
Biochain |
50 ug |
EUR 351 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from hypertension: Heart |
|
R1236122Hd-2 |
Biochain |
50 ug |
EUR 351 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Lupus: Kidney |
|
R1236142Lup-50 |
Biochain |
50 ug |
EUR 351 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Lupus: Liver |
|
R1236149Lup-50 |
Biochain |
50 ug |
EUR 351 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Lupus: Lung |
|
R1236152Lup-50 |
Biochain |
50 ug |
EUR 351 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Lupus: Pancreas |
|
R1236188Lup-50 |
Biochain |
50 ug |
EUR 351 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Lupus: Spleen |
|
R1236246Lup-50 |
Biochain |
50 ug |
EUR 351 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Lupus: Stomach |
|
R1236248Lup-50 |
Biochain |
50 ug |
EUR 351 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 AnaPrep Total RNA Extraction Kit |
|
Z1322015 |
Biochain |
1 kit (48 extractions) Including all required plastic disposables |
EUR 308 |
|
Description: This kit is developped for our fully automated magnetic bead-based nucleic acid extraction platform which uses preprogrammed protocols and can process up to 12 samples simultaneously. With the AnaPrep 12 Extractor you will have the option to choose and work with a wide range of sample and reagent volumes. This sturdy, realiable and user-friendly machine will save you both time and expenses while ensuring consistently high quality performance and nucleic acids for your downstream applications. |
 Uterus Lysate |
|
1317 |
ProSci |
0.1 mg |
EUR 191 |
|
Description: Uterus tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
 Total RNA from Rat Normal Tissue: Brain, cerebellum |
|
R1434039-50 |
Biochain |
50 ug |
EUR 152 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Skeletal Muscle |
|
R1434171-50 |
Biochain |
50 ug |
EUR 152 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Total RNA from Rat Normal Tissue: Small Intestine |
|
R1434226-50 |
Biochain |
50 ug |
EUR 214 |
|
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
) Plant Total RNA Mini Kit (50prep) |
|
FAPRK-001 |
Favorgen |
50 preps |
EUR 165 |
) Plant Total RNA Mini Kit (100prep) |
|
FAPRK-001-1 |
Favorgen |
100 preps |
EUR 216 |
) Plant Total RNA Mini Kit (300prep) |
|
FAPRK-001-2 |
Favorgen |
300 preps |
EUR 404 |
) Plant Total RNA Maxi Kit (10prep) |
|
FAPRK-002 |
Favorgen |
10 preps |
EUR 201 |
) Plant Total RNA Maxi Kit (24prep) |
|
FAPRK-002-1 |
Favorgen |
24 preps |
EUR 317 |
) Tissue Total RNA Mini Kit (50prep) |
|
FATRK-001 |
Favorgen |
50 preps |
EUR 165 |
) Tissue Total RNA Mini Kit (100prep) |
|
FATRK-001-1 |
Favorgen |
100 preps |
EUR 216 |
) Tissue Total RNA Mini Kit (300prep) |
|
FATRK-001-2 |
Favorgen |
300 preps |
EUR 404 |
) Tissue Total RNA Maxi Kit (10prep) |
|
FATRK-003 |
Favorgen |
10 preps |
EUR 201 |
) Tissue Total RNA Maxi Kit (24prep) |
|
FATRK-003-1 |
Favorgen |
24 preps |
EUR 317 |
 Rapid Animal Total RNA Extraction Kit |
|
AT4181 |
Bio Basic |
50Preps |
EUR 88.06 |
|
|
Rapid Animal Total RNA Extraction Kit |
|
AT4182 |
Bio Basic |
250preps |
EUR 202.25 |
|
|
 Total RNA ExtractionMini KitBlood/Cultured Cell |
|
FYG301-300P |
Yeastern Biotech |
300 Preps |
Ask for price |
 Zenoquick Whole Blood Total RNA Kit |
|
Z5010-050 |
GenDepot |
50 preps |
EUR 482 |
Zenoquick Whole Blood Total RNA Kit |
|
Z5010-100 |
GenDepot |
2x50 preps |
EUR 806 |
 Broad Range Total RNA Isolation Kit |
|
K1341050 |
Biochain |
1 kit |
EUR 266 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
As well as, Sre1 direct goal genes have been recognized by way of ChIP mixed with lambda exonuclease digestion (ChIP-exo) assays. SRE motifs have been detected within the promoter areas of a number of Sre1 direct goal genes and have been in line with the SREs described in different yeast species. Sre1 immediately regulates genes associated to ergosterol biosynthesis in addition to genes associated to the mevalonate (MVA) pathway, which synthesizes the constructing blocks of isoprenoids, together with carotenoids. Two carotenogenic genes, crtE and crtR, have been additionally recognized as Sre1 direct goal genes.
